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KMID : 0603520000050030154
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Journal of Korean Association of Cancer Prevention
2000 Volume.5 No. 3 p.154 ~ p.159
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Neutrophil-Mediated Oxidative Activity of Coccoid Forms of Helicobacter pylori
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Hong Weon-Seon
Kim Hea-Won
Lee Hak-Sung
Myung Seung-Jae
Jung Hwoon-Yong
Yang Seok-Kyun
Kim Jin-Ho
Min Young-Il
Chung Myung-Hee
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Abstract
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Helicobacter pylori (H. pylori) is now recognized as the major etiologic agent of chronic gastritis and plays an active role in the pathogenesis of peptic ulcer and gastric cancer. One of the characteristic features of H. pylori infection is the infiltration of neutrophils and their production of oxygen-derived free radicals (OFRs) in gastric epithelium.
H. pylori exists in two forms, spiral and coccoid form. Although spiral form is known to cause the mucosal injury by increased OFRs, it is still unclear whether coccoid form causes the OFR-mediated mucosal injury. Coccoid forms were induced by incubating spiral forms of two strains of H. pylori, ATCC 43504 (NCTC 11637) and DU-3, a clinical
isolate from a patient with duodenal ulcer, in Brucella broth media for 84 hr. Spiral forms were obtained after 36 hr incubation. The levels of OFRs were determined by luminol-dependent chemiluminescence (ChL). There was no significant difference in the production of ChL between spiral and coccoid forms of ATCC 43504 and DU-3. On the
other hand, the heat-killed H. pylori did not activate neutrophils to produce OFRs. These results suggest that not only spiral form but also coccoid form may contribute to OFR-mediated gastric mucosal injury.
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KEYWORD
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pylori, Spiral form, Coccoid form, Oxygen-derived free radicals
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